Extrato aquoso de folhas de Vernonanthura polyanthes: angiogênese e toxicidade

Abstract

The specie Vernonanthura polyanthes popularly known as assa-peixe, belongs to the family Asteraceae and presents pharmacological properties in function of the bioactive compounds that it possesses. Popularly it has been used to treat a variety of diseases, including wounds, bronchitis, pneumonia, hemoptysis, persistent cough, internal abscesses, gastric pain and kidney stones. In order to evaluate the potential of the aqueous extract of V. polyanthes extract the objectives of the present study were: 1) to identify angiogenic activity; 2) perform a scientiometric analysis to identify trends in the use of chicken chorioallantoic membrane (CAM) technique and 3) evaluate the toxic and cytotoxic potential. For analysis of angiogenic activity the CAM assay was used. For this analysis, the following treatments were used: sterile water, Regederm, dexamethasone and the leaves extract of V. polyanthes at 10, 20 and 40 mg/ml concentrations. As a result, concentrations of 20 mg/ml and 40 mg/ml have antiangiogenic properties. These results suggest the use of this plant as a possible therapeutic agent that can be used for the treatment of tumors. The sciciometric analysis was done by searching for scientific articles in the ISI - Web of Knowedge using the following combinations of words: "chorioallantoic membrane" and CAM, "chorioallantoic membrane" and "chick embryo membrane". The results showed that the CAM technique is a reliable method, which has been widely used by the scientific community to respond different biological processes. In order to evaluate the toxic and cytogenotoxic activity of the aqueous extract of the leafs from V. polyanthes, the methods of Artemia salina and Allium cepa were used. As a result the aqueous extract of leafs from V. polyanthess prepared in the concentrations of 20, 40 and 80 mg / ml were toxic to Artemia salina, with the LC50 toxicity threshold being 24 mg / ml. Also in relation to toxicity, the A. salina showed toxicity of V. polyanthes extracts for all concentrations tested (10, 20 and 40 mg/ml). Microscopy analyzes of the A. salina showed that aqueous extracts of V. polyanthes leaves are cytotoxic in the concentration of 40 mg/ml. No concentration studied showed significant genotoxicity in the cells of A. salina.