Estudos de metabólitos secundários produzidos por aspergillus flavus e paecilomyces variotii isolados do solo do cerrado

Abstract

Microorganisms are an important source of bioactive compounds with high economic value and many advantages concerning their use and production. The objective of this study was to isolate fungi from Cerrado soil and identify those with a promising source of natural bioactive compounds, precisely because they have such a structural diversity. This, together with the wide range of microorganisms we can find in nature, particularly those that have not been exploited, allows us to manipulate the metabolic pathways of culture of the fungi. To begin with, fungi from the Cerrado soil in the state of Goiás were isolated and selected considering their potential production of secondary metabolites with biological activity. The selection of 11 isolated fungi happened previously due to the results of toxicity to Artemia salina and High-Performance Liquid Chromatography. Two species of fungi had had promising previous results compared to the other ones. These fungi were identified from the extraction of their DNA and amplification of their 18S rRNA gene, which featured Aspergillus flavus and Paecilomyces variotii . The compounds were extracted from these fungi, followed by an evaluation of the biological potential of the compounds produced by fungi A. flavus and P. variotii through the level of toxicity to Artemia salina, antimicrobial susceptibility testing by disk diffusion and dilution and the minimum inhibitory concentration and cytotoxic evaluation by the MTT method. Chemical analyses of the compounds were done through High-Performance Liquid Chromatography (HPLC) and High-Performance Liquid Chromatography combined to Mass Spectrometry (HPLC - MS / MS) in order to elucidate the compounds. Through this study, it was possible to check the potential of the Cerrado soil in Goiás when it comes to providing fungi, as well as the use of compounds produced by A. flavus and P. variotii with industrial interest.