Atividade de nanopartículas Fe3O4/Ag na formação de biofilme por pseudomonas aeruginosa

Abstract

Pseudomonas aeruginosa are recognized for their pathogenicity, resistance to antimicrobials, and ability to grow in biofilms, which makes them even more problematic in healthcare. In this context, nanotechnology allows the development of new materials against multi-resistant microorganisms. In the present work, colloidal dispersions of iron oxide nanoparticles (IONPs), silver (AgNPs), and iron oxide/silver composite (IO/Ag-NCs) were prepared and tested for toxicity and activity against biofilms of Pseudomonas aeruginosa clinical isolates. The heterodimer nanocomposites showed spherical morphology and zeta potential, indicating relative colloidal stability despite their polydispersity. The nanoparticles showed no toxicity to Artemia salina at the concentrations tested and inhibited biofilm formation of some of the clinical isolates of P. aeruginosa. The NPs inhibited it for three isolates of P. aeruginosa. Our results indicate that further research should be directed toward strategies that consider increasing silver concentration and inhibited the biofilm formation of some of the clinical isolates of P. aeruginosa. AgNPs inhibited biofilm formation for three isolates, IONPs reduced it for four, and IO/Ag-NCs using IO/Ag-NCs as a nanocarrier for controlling microbial biofilm formation.